#10 DNA Sequencing and Mutagenesis

the most widely used DNA sequencing method

Two general methods used for sequencing

Which sequencing method uses chemical degradation of the original DNA

The _________ sequencing method is used to confirm the _______ sequencing method

Which sequencing method is used for analysis of DNA modifications

The Sanger Method uses a ____________ to terminate chain elongation at a specific nucleotide

This mutagenesis method uses a primer that has the appropriate mismatch located within it

Oligonucleotide-mediated mutagenesis requires what 3 reagents

Allows one to insert a small sequence of altered bases into a sequence

compression of the sequence occurs as the replicated DNA gets

Which sequencing method readily gives nucleotide base sequences but not modifications

sequencing details read in what direction

This mutagenesis method is limited to the mutation of just a few nucleotides at a time

This mutagenesis method uses a universal primer

PCR-mediated mutagenesis uses a ____________ that has the appropriate mismatch located within it

most DNA sequencing uses the ________ method

Sanger cannot sequence areas that induce _______

Oligonucleotide-mediated mutagenesis prefers _________ DNA over __________ DNA.

___________ changes DNA sequences at a chosen location

The flanking region has a sequence that is __________ to the ______________