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Micro Exam 2
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Metabolism
All biochemical process in a cell
Catabolism
Breaking down molecules
exergonic reaction
energy releasing
Anabolism
building up molecules
endergonic reaction
energy requiring
ATP
grabs energy released during exergonic reactions then holds onto enegry to use during endergonic.
Free Energy Change ⌂G
0,
the energy released that is available to do work
Amout gain or lost in a reaction
Exergonic is a negative value
Endergonic is a positive value
Energy
Compacity to do work
potential or kinetic
A.T.P
Adenosine Triphosphate
Calories
Amount of heat necessary to raise temperature of 1 gram of water from 14.5C to 15.5C
Laws of Thermodynamics
Energy is not created or distroyed
Some energy is always lost when converted from one form of energy to another form (entropy)
N.A.D.
Nicotinamide-adenine Dinucleotide
F.A.D
Flavin Adenine Dinucleotide
Color produced on Psuedo F
Fluoresein - yellow
Color produced on Pseudo P
Pyocyanin - blue/green
First Stain used in the Ziehl-Neelsen Acid Fast Stain
Carbol Fusion
What is in the Acid Decolorizer
Hydrocloric Acid and Ethylnal
What is the counterstian used in Acid - Fast Staining
Methylene Blue
What is the first stain used in the Schaeffer-Fulton staining technique for endospores
Malachite green
What is the stain used in the optional staining technique for endospores
Safranin
What is the second stain used in the Schaeffer-Fulton staining technique
Safranin
What does N.A.D do
it can accept both electrons and protons in order to become stable
How do you get NADH
NAD accepts 2 electrons and 2 protons, causing it to become more stable and reduce
Enzymes
Biological catalysts
proteins
lower engery needed for activation
hold reactants in a certain way to start reaction
consintrate reactants against gradient
What is activation energy
amount of energy need to start a reaction
the energy required to bring all molecules in a chemical reaction into the reactive state
Fermentation
is the least effective form
Photophosphorylation
H+ on one side of the membrare
light engery does all the work pumping protons
occurs in phototrophic organisms
ATP is synthesized during photosynthetic electron flow from the activity of ATPase that couples the proton motive froce to ATP formation
In what kind of organisms is O
2
the terminal external electron receptor
Aerobic
S, NO
3
-
, SO
4
3-
are all external terminal electron receptors for this type of complete oxidation of organic compounds
Anarobic
Descirbe substrate level phosphorylation
ATP is synthesized directly from an energy rich intermediate during steps in the catabolism of fermentable compounds
only type fermentors can use
Oxidavite Phosphorylation
Also known as the electron transport phosphorylation
ATP is produced at the expense of the proton motive force
Anhydride Bonds
Stores lots of enegry
enzymes which is either ATPsyntase/ATPase locks negative charges together
Exergonic
enegry yielding reactions
energy releasing reactions
Endergonic
reactions that require energy
Catalyst
a substance that lowers the activation energy of a reaction, thereby increasing the reaction rate
Oxidation
the removal of an electron or electrons from a substance
Reduction
the addition of an electron or electrons to a substance
Psychrophiles
organisms with a low temperature tolerance
Author
lledbetter.14
ID
133922
Card Set
Micro Exam 2
Description
Micro Exam 2
Updated
2012-02-10T02:50:07Z
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