Micro Lab 1

Purpose: to determine the bacteria cell shape.

-use clean slide apply 1-2 drops of distilled water to slide with flamed inoc. loop.

-reflame the loop, transfer small amout of bacteria from medium surface (with water on slide.)

-spread bacteria, water suspension into thin film which covers 3/4 slide surface; reflame loop.

-allow smear to air dry completely

• -heat "fix" smear per oral instruction.
• (make bacteria stick to slide so it wont wash off with water. also it will kill it.)

• basic stain: Methylene Blue
• 

Bacillus

*most important tech.

Purpose: A differential staining technique which separates bacteria into two groups: Gram Postive and Gram Negative.

• -apply primary stain: Crystal Violet (3min)
• -rinse
• -apply mordant: Gram's Iodine (2min)
• -rinse
• -apply decolorizing agent: Ethyl Alcohol
• -rinse
• -apply counter stain: Safranin (1min)

• Gram (+) bacteria:
• it will look purple because there are small amount of lipid in the cell wall.

• Gram (-) bacteria:
• it will look red because there are large amount of lipid in the cell wall.

• Bacillus subtillis (+) rods
• Streptococcus Faecalis (+) circles
• Eschericha Coli (-) rods
• Neisseria Perflava (-) circles
• Moraxella Brahamella (-) circles

Reaction: positive (+) so it should be purple and rods.



ha there are a mix of bac and coc in there. somehow...?

Reaction: negative (-) should be purple and circles.