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Microbiology
The study of living things to small to be seen without magnificatoin
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microorganisms
germs or viruses, microsopic organisms
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viruses
noncelluar, parascitic, protein-coated gentic elements that can infect other living things
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organisms without a true nucleus
prokaryotes
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organisms with a true nucleus
eukaryotyes
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ubiquity
found everywhere, large numbers
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bacteria preformed this long before plants
photosynthesis
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way humans use bacteria
bake, cheese, wound healing, beer
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biotehnology
humans manipulate microorganisms to make products in an industrail setting
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genetic engineering
GMOs, create new products and genetically modify organisms
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Recombinante DNA technology
allows microbes to be engireered to synthesize disireable proteins(i.e. drugs, hormones, and enzymes)
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Bioremediation
- Øintroducing microbes in to the environment to restore stability or clean up toxic pollutants
- Oil spillsl
- Chemical spillsl
- Water and
- sewage treatment
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Pathogens
Ødisease-causing organisms
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Other diseases previously not linked to microorganisms now are
gastric ulcers, certain cancers, schizophrenia, multiple sclerosis, obsessive compulsive disorder, coronary artery disease)
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lProkaryotic vs. eukaryotic cells
•Prokaryotic cells are about 10 times smaller than eukaryotic cells•Prokaryotic cells lack many cell structures such as organelles •All prokaryotes are microorganisms, but only some eukaryotes are
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ØViruses
Not independently living - acellular organismsØMuch simpler than cells- basically a small amount of DNA or RNA wrapped in protein and sometimes by a lipid membraneØIndividuals are called a virus particle or virion ØDepend on the infected cell’s machinery to multiply and disperse
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Robert Hooke
Coined the term cell
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Joseph Lister
aseptic technique
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Louis Pasteur
•Pasteurization•The Germ Theory of Disease•Vaccination
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lRobert Koch
verified the germ theory
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What is the germ theory of disease?
lMicrobes (germs) cause disease and specific microbes cause specific diseases.
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. Who proved this theory?
lRobert Koch in the late 1870's.
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Ø3. What disease was he studying?
lanthrax - disease of cattle/sheep
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Ø4. What was his experimental method?
lHe observed that the same microbes were present in all blood samples of infected animals. He isolated and cultivated these microbes (Bacillus anthracis ). He then injected a healthy animal with the cultured bacteria & that animal became infected with anthrax & its blood sample showed the same microbes as the originally infected animals.
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Ø5. What did his experiments prove?
lParticular microbes cause particular diseases.
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Ø6. What are Koch's 4 Postulates?
l1. The causative agent must be present in every individual with the disease.l2. The causative agent must be isolated & grown in pure culture (how did he invent pure cultures? with Frau Hesse's help, he developed the agar plate method.)l3. The pure culture must cause the disease when inoculated into an experimental animal.l4. The causative agent must be reisolated from the experimental animal & reidentified in pure culture.
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Microbial nomenclature-
naming microorganisms
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Taxonomy
classifying living things
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binomial system of nomenclature
genus name followed by the species name
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Phylogeny
the degree of relatedness between groups of living things
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evolution- Two preconceptions:
- •All new species originate from preexisting species
- •Closely related organisms have similar features because they evolved from a common ancestor
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