Genetics 6

  1. Methylates adenine
    Mut S
  2. Cuts within the GATC site
    a) What type of activity and
    b) works along side who?
    • Mut H
    • a) Endonuclease activity
    • b) Mut L
  3. Binds to the mismatch
    Mut H
  4. Methyl directed mismatch repair is done via the presence of what site?
    GATC site
  5. Promoter is found on this strand
    Coding strand
  6. Recognizes -35 and -10 promoter
    Sigma subunit
  7. DNA is opened how much and forms what?
    18 bp, and forms transcription bubble
  8. Section on RNA polymerase which carries out polymerization
    Core enzyme
  9. Two types of termination of transcription
    a) A DNA-RNA helicase which requires ATP
    b) Inverted repeats in DNA which form secondary sequences
    • Rho dependent termination and Rho independent determination
    • a) Rho Protein
    • b)Stem Loops
  10. Three eukaryotic RNA polymerases
    a) Main transcribing enzyme
    b) Transcribes 5sRNA and tRNA
    c) Transcribes DNA in nucleolar organelle and rRNA
    • I, II, III
    • a) II
    • b) III
    • c) I
  11. Location of mRNA synthesis
  12. Location of translation
  13. Promoter for eukaryotes (2 Names)
    a) located hor far upstream or downstream?
    • TATA Box or Hogness Box
    • a) -25 bp upstream
  14. Initiation requires what?
    a) Binds to TATA box (has a transcription binding protein)
    b) Bind to give initiation complex
    c) Has helicase activity
    • Transcription factors
    • a) TFIID
    • b) RNA pol & other transcription factors
    • c) TFIIH
  15. Encodes more than one gene
  16. Omtervening sequences that are not part of the gene
  17. Initial RNA transcript
  18. 3 Processing steps of initial RNA transcript
    • 5’ cap of 7-methyl guanine,
    • 3’ poly-A tail via polyadenylation,
    • and removal of introns & splicing of exons
  19. Process which adds a chai of anenine to stabilize RNA
  20. Process of joining exons
  21. Large RNA-Protein complex
    a) RNA portion
    b) Protein portion
    • Spliceosome
    • a) snRNA
    • b) snRNP
  22. Cause insertions
  23. Three different codons for the same amino acid
  24. Opposite of the codon sequence
  25. Creates charged tRNA
    Amino acyl tRNA synthetase
  26. 70S ribosome of bacteria contains what two subunits
    a)50S contains what two rRNAs
    b)30S contains what rRNA
    • 30S and 50S
    • a) 23S rRNA and 5S rRNA
    • b) 16S rRNA
  27. Unit of centrifugation
  28. Direction of protein synthesis
    N to C
  29. Start codon
    a)Codes for ___in prokaryotes
    b)Codes for__in eukaryotes
    • AUG
    • b) Formyl Methionine
    • c) Methionine
  30. Area to which 30S binds to begin translation in prokaryotes
    a) recognized by what in the 30S
    • Shine Delgarno sequence
    • a) 16S rRNA
  31. Area for eukaryotes to being translation
    5' cap
  32. EPA sites are found in which subunit
    50S subunit
  33. Initiation requires these
    a) Bring 30S to Shine delgarno sequence
    b) Bring tRNA charged with formyl methionine (requires GTP)
    c) 50S binds with AUG in what site?
    • Initiation factors
    • a) IF1 and IF3
    • b) IF2
    • c) P site
  34. Forms peptide bonds
    a) Location
    • Peptidyle transferase
    • a) 50S subunit
  35. Ribosome with enzymatic activity
    Peptidyl transferase
  36. Brings charged tRNA
  37. Translocates ribosome
  38. Polypeptide=330 amino acids why?
    Due to 1000 bo of a gene in prokaryotes
  39. Stop Codons
  40. Pulls out polypeptide to allow ribosome to dissociate
    Release factor
  41. Chemical or factor which causes mutation
  42. Mutation without a mutagen
  43. When mutagen induces a specific mutation
  44. Mutation dependant on environment
    a) Mutation expressed
    b) Wild-type expressed
    • Conditional Mutant
    • a) Restrictive
    • b)Permissive
  45. Purine to Purine or Pyrimidine to Pyrimidine
  46. Purine to pyrimidine or pyrimidine to purine
  47. Area which has cytosine
  48. Deamination of cytosine produces this
  49. Takes out uracil
    Uracil glycosylase
  50. Mutation which results in one different amino acid
  51. Mutation which resuslts in same amino acid, but different sequence
  52. Mutation which results in a stop codon
  53. Mutations which causes codons to shift
    a) Detrimental & not leaky because it produces a nonfunctional polypeptide
    Framshift muation
  54. Fit between bases and cause frameshift mutations
    a) Example of one
    • Intercalating agents
    • a) Acradine Orange
  55. Causes cyclobutane ring between ____-____ (known as ____)
    Ultraviolet radiation, Thymine-Thymine, known as Thymine Dimers
  56. Binds to cyclobutane ring and breaks
    a) Occurs at what wavelengths?
    • Photolyase
    • a) 300-500nm
  57. Sugar is a carbon source which is catabolic and ...
    the cell does not synthesise these
  58. Gal- means
    the cell needs galactose for growth
  59. F+ During conjugation is the
    The F factor
  60. Hfr would...
    be the host itself or would carry the gene during conjugation, donates genes from bacteria chromosomes, has F factor inserted in bacteria chromosome, may become F+ cell, and rarely transfers the F factor to other cells
  61. Proofreading is due to
    3' to 5' exonucleases
  62. What stops transformation
    Adding DNase
  63. What is the main replicating enzyme
    Polymerase III
  64. 3' to 5' template gives
    Continuous replication
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Genetics 6
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