-
What is a monocular microscope?
- one eye piece
- less expensive
-
what is a dissecting microscope used for?
larger objects because it only magnifies 30-90x
-
what kind of microscope do we use?
binocular light microscope
-
how do you pick up your microscope?
one hand on the handle and the other hand on the bottom
-
what should you do before using the microscope?
- clean lenses
- clean oculars
- wipe lenses dry
-
how do you clean the lenses?
wipe any oil from objective with cotton-tipped applicator with alcohol. clean all objectives
-
what material can you use to wipe clean the lenses and oculars?
dry cotton-tipped applicator or lens paper
-
what are the different types of lights?
- tungsten (less expensive)
- halogen (brighter, more expensive)
-
what is an ocular?
eye piece
-
how much does an ocular magnify?
10x
-
what is interpupillary distance?
the distance between your eyes
-
what are the different objectives?
-
which objective do we use for oil immersion?
100x
-
which objective is the scanning objective?
4x
-
which objective is low power?
10x
-
which objective is high power?
40x
-
what is magnification?
ocular x objective
-
what is the mechanical stage?
holds the slide with stage clips
-
how can you move the stage?
with coarse and fine focus knobs
-
what is the condenser?
focuses light on the object
-
what will happen if you move the condenser dow?
it will increase the depth of field
-
what happens to the brightness of the field when you move the condenser down?
decreases the brightness
-
what is the iris diaphragm?
alters the amount of light reaching the object
-
where is the iris diaphragm located?
within the condenser
-
what happens when you open the iris diaphragm?
increases the amount of light
-
what happens when you close the iris diaphragm?
decreases the amount of light
-
what objective should you start with when viewing a slide?
10x
-
whare are the steps to the correct use of the microscope
- turn it on
- set interpupillary distance
- place slide on stage between clips
- start with 10x objective
- bring into focus
- adjust condenser and diaphragm to give depth of field and correct light penetration
-
how do you bring a slide into focus?
start with the stage in highest position, move stage down until the slide comes into focus
-
what are the three ways to adjust the amount of light?
- turn rheostat up
- open diaphragm
- move condenser up
-
do unstained objects need more light or less light?
less light
-
if there is too much light, what should you do?
- turn down rheostat
- close diaphragm
- drop condenser down
-
what should you do when you have the scope on low power (10x)?
evaluate: staining if stained, cellularity, distribution
-
what do you do for fecals?
scan the entire slide at low power
-
how do you focus a slide when it's on 40x or 100x?
using the fine focus knob only!!
-
when do you use oil immersion?
blood smears and cytology
-
how do you use oil immersion?
- place drop of immersion oil on slide
- move 100x objective into place
- adjust using the fine adjustment only!
-
how big is the average dog RBC?
7 microns
-
describe low power
- see large field of view
- objects only magnified 100x
- see less detail
- requires less light than higher power
-
describe high power
- smaller hole
- smaller field of view
- more light needed
- objects appear larger
- total magnification is 400x
-
what do you do when you are ready to put away your microscope?
- wipe oil from objective with cotton-tipped applicator with alcohol
- clean all objectives
- wipe objectives dry using dry cotton-tipped applicator or lens paper
- clean oculars
- place 4x objective down
- unplug, fold cord, stage down to hold cord
- place dust cover over scope and put away
-
Defin ocular lens
eye piece
-
Define objective lens
the part that magnifies either 4x, 10x, 40x, or 100x
-
Define mechanical stage
the part where the slides sits and can move up and down to focus
-
Define stage clips
holds the slide in place
-
Define course focus
moves the mechanical stage up and down...only move in 4x or 10x
-
Define fine focus
moves the stage up and down to focus...move at 40x or 100x
-
Define condenser
- focuses light on the object
- increases or decreases the depth of field
- increases or decreases brightness
-
Define iris diaphragm
controls how much light reaches the object...it within the condenser
-
-
Define stage control knobs
moves the stage sideways
-
Define rheostat
controls light
-
Define power switch
turns on the microscope
-
List three ways to increase the amount of light passing through the slide
- turn up the rheostat
- raise the condenser
- open the iris diaphragm
-
List two things that affect the amount of light passing through the slide.
- stained or unstained
- thickness
-
What materials can be used to clean lenses and objectives?
- dry cotton applicator (q-tip)
- lense paper
-
On what power is it okay to use the course adjustment knob?
10x
-
What is meant by inter-pupillary distance?
the distance between the eyes
-
What's a micrometer?
- 1,000th of a millimeter
- the measurement we use when using the microscope
-
How can a red blood cell be used in lieu of a micrometer?
a dog's RBC is 7 micrometers so we use that as a comparison to the other RBC's
-
What happens to the field of view as you change from a 10x objective to a 40x objective? What about vice versa?
the field of veiw decreases
-
Which objective should you use immersion oil?
100x
-
Which part of the microscope controls the depth of field?
condenser
-
What is the total magnification achieved with a 10x ocular and a 40x objective?
400x
-
How should the immersion oil be cleaned from the microscope?
with alochol and a dry cotton applicator or lense paper
-
What is the purpose of the condenser?
- changes depth of field of view
- regulates the light reaching the slide
-
How should the condenser be set for viewing a wet prep of urine?
low
-
How should the condenser be set for viewing a fecal float?
low
-
How shold the condenser be set for viewing a dry-prep of a blood smear?
high
-
What effect will moving the condenser downward have on the brightness you see?
decreases the brightness
-
What does the iris diaphragm do?
regulates the amount of light
-
How do you properly put the microscope away?
- clean oculars and objectives
- make sure it is set on the 4x objective
- put the stage all the way down
- wind up the cord
- place dust cover over the microscope
- store it in the cabinet
-
What is the major benefit of halogen lights
they are brighter and have truer light
-
What is the major advantage of tungsten lights?
cheaper
|
|
