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Give 7 general characteristics of NF:
- 15% of the clinical isolates
- Ubiquitous
- Environment (plants, soil, water)
- Dialysis fluids, saline, A lines, catheters
- Opportunistic
- Gram =
- Motility (flagella diff types)
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What types of specimens can be collected for NF?
- Blood
- Wound
- Respiratory tract
- Foreign body implantation
- Infused fluids
- Immunocommpromised patients (differ sites)
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What types of disease can be associated to NF Bacteria?
- Diabetes
- Cancer
- Steroid treatment
- Transplantation
- Catheters (prosthetic devices)
- Dialysis patients
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What are the Growth Characteristics of NF?
- Colony size is variable
- Pigment
- Hemolytic
- 35 and 25
- Aerobes
- Utilize Entner-Dudoroff Pathway
- Oxidize CHO
- Slow growers
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What are the KEY characteristics for clinical diagnosis of NF?
- No acid production from CHO
- TSI K/K
- Growth on Mac
- Motility
- Oxidase + but may vary
- Slow grower
- Oxidizer
- Resistant to various antibiotics (aminoglycosides, cephalosporins, fluoroquinolones
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What are the 3 types of Motility?
- Polar
- Bipolar
- Lophotrichous
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What organisms are Motile Saccharolytic?
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What organisms are Motile Non-Saccharolytic?
Alcaligenes faecalis Achromobacter Pseudomonas alcaligenes P. pseudoalcaligenes Shewenella
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What are 3 clues that tell you an organism is a NF?
- No glucose fermentation
- Oxidase +
- No growth on Mac
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What are the main biochemicals for NFs?
- OF
- Nitrate
- Growth on Mac
- Motility
- Indole
- Urea
- Esculin hydrolysis
- Pigment
- Decarboxylation
- H2S
- Temperature
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What are the Medically Important Organisms for NFs?
- Acinetobacter
- Burkholderia
- Chrysobacterium
- Flavobacterium
- Moraxella
- Pseudomonas
- Stenotrophomonas
- Shewenella putrefaciens
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Give characteristics of P. aeruginosa:
- Pyocyanin +
- Fluorescin +
- Pyoverdin +
- Oxidase +
- Growth @ 42 C
- Motile
- Mac +
- Resistant to various antibiotics
-
Give characteristics of P. fluroescens:
- Oxidase +
- Fluroescin +
- Pyocyanin =
- Growth 42 C =
- Motile
- Mac +
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Give characteristics of Stenotrophomonas maltophilia:
- Oxidase +
- Maltose +
- Esculin +
- DNase +
- Mac +
- Motile
-
Give characteristics of Shewenella putrefaciens:
- Oxidase +
- DNase +
- H2S +
- Mac +
- Motile
-
Give characteristics of Acinetobater:
- Mac +
- Oxidase =
- Non-motile
- Coccobacilli
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Give characteristics of Alcaligenes faecalis and Pseudomonas alcaligenes :
-
Give characteristics of Moraxella:
- Mac =/+
- Oxidase +
- Non-motile
-
Give characteristics of Cryseobacterium and Meningocepticum:
- Mac +
- Indole +
- Oxidase +
- DNase +
- Non-motile
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Pseudomonas aeruginosa infections:
- Bacteremia (6%)
- Endocarditis
- Pulmonary Infections
- Ear Infections
- Burn wound infections
- UTI
- Gastroenteritis
- Eye infection
- Nonsocomial (75%)
- Cystic fibrosis
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What are the Virulent factors for P.aeruginosa?
- Proteases
- Exotoxin A
- Phospholipase
- Capsule (CF patients)
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