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Hypothesis
an educated guess based on available information
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Null hypothesis
the opposite of the hypothesis
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Theory
a hypothesis with a large body of evidence to support it
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Law
a well-tested theory that has become an immutable fact
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What are experiments designed to do?
to determine if the null hypothesis can be refuted
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Variable
a condition that exists in more than one possible state, e.g. temperature,pH, exposure to sunlight etc.
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Dependent
variables that must be held constant in an experiment
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independent
variables that are allowed to fluctuate, the independent variable is the subject of the experiment only one thing.
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control group
a portion of the experiment that does not receive a treatment, this shows a baseline for comparison with the portion of the group that receives the treatment
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placebo
a sugar pill used in drug treals, an individual in the trial will be unaware if they are receiving the treatment or a placebo to eliminate psychosomatic vareables
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placebo effect
some test subjects will respond to a placebo because they are told it is an actual treatment
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double-blind study
the experimenter(s) do not know whether an individual subject is getting the treatment ora placebo unti9l the study is complete, this eliminates experimenter bieas as a variable.
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8 characteristics of life on earth
- 1.cellular
- 2.Dna based
- 3. Reproduction
- 4. Growth
- 5. Metabolism=use of energy
- 6.Homeostasis=maintaining a steady state
- 7. Evolution=adapt to environment
- 8. Carbom/water based=organic molecules
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atomic number
the number of protons
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atomic mass
the number of protoms and neutrons
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isotopes
atoms with the same number of protons but different numbers of neutrons
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ionic
one atom takes an electron from another atom to create an elctrical attraction
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covalent
two atoms share electrons to create a bond
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cohesion
water molecules "stick" to each other
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adhesion
water molecules form hydrogen bonds with other molecules
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acids
chemicals that cause the pH of water to decrease
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bases
chemicals that cause the pH of water to increase
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Buffers
solutions that have chemicals in them that resist changes in pH
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examples of oganic elements
C H N O P & S
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Biomolecules
Carbohydrates, proteins, Nucleic acids,Lipids
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monosaccharide
single sugar molecule
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Disaccharide
two linked sugar molecules
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oligosaccharide
a small chain (3-100) of sugar molecules
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polysaccharide
a long chain of sugar molecules, e.g. starch,glycogen,cellulose
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carbohydrate functions
energy storage, structural, in plant and bacteria cell walls
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Dipeptide
two linked amino acids
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proteins are polymers of amino acids
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polypeptide
a chain of amino acids
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Primary
the sequence of amino acids
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Secondary
localized structure comsisting elements such as the alpha- helix and beta-pleated sheet
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tertiary
the three dimensional structure of a single polypeptide
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quaternary
the total proteirn shape that inludes multiple polypeptides
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Energy
the capacity o do work
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potential energy
energy of position
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kinectic energy
energy of motion
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Thermodynamics Law 1
energy can neither be created nor destoryed it can only change forms
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thermodynamics law 2
anytime energy changes form some energy is lost as heat
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metabolism
the sum total of all chmial reaction in a cell or organism
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endergonic
the reaction requires an input of energy
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exergonic
the reaction releases energy
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chemical equilibrium
some chemical reations move in both the forward and reverse directions
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active site
this is the part of the encyme where the chemical reaction occurs.
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enzymes
are proteins that have large complicated structuresEncymes grab the reactants and position them so that they will react to form products. After the product are formed the enzyme releases them the suffix- ase
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substrates
reactants in the enzyme ataized reaction
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Activation energy
- energy that must be added to a reation to get it started this applies to exergonic reactions as well as endergonic
- enzymes work by lowering the activation energy of a reaction, this makes the reaction go faster however it does not effect the equilibrium
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cofactors
non-protein chmicas found in the active site that faciitate function
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NEGATIVE feedback
the end product of a series of reactions inhibits the first enzyme in the series
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pOSITIVE feedback
the end product of a series of reactions activates an enzyme in the series
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cell cycle phases
Phases a cell undergoes cell division and replication of DNA
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G1 first phase
The first growth phase is when the cell carries on anabolism to prepare the cell for synthesis and subsequesnt division.
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G0 phase
if a cell does not need to divide it stops early in the G1 phase to enter G0 where it will not divide.
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S phase
the synthesis phase is when the cell replicates in DNA
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G2 second growth phase of cell
during the second growth phases the cell prepares for division by condensing the DNA and assembling the proteirns need for diviosn.
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Mitosis
the separating of the two identical copies of DNA
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cytokinesis
the physical separation of a single parent cell into two daughter cells.
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Synthesis Phase
In order for the cell to divide it must duplicate in DNA in a process called replication,it is important to know that double stranded DNAe is anti-parallel
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How do enzymes help in the Replication of DNA?
Denzymes carry our replication of DNA in a specific sequences.
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Mitosis is a four step process
1. Prophase
2. Metaphase
3.Anaphase
4.Telopase
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DNA Helicase
opens up the double helix by breaking the hydogen bonds
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single stranded binding proteins
bind to the single stranded DNA to prevent it from reforming the double helix
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RNA Primase
adds a short sequence of RNA to begin the duplication process
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DNA Polymerase
synthesis the DNA polymer from free nucleotides, the nucleotides that are added must pair with the existing strand according to chargraff's rule. DNA can only wrk in 3 to 5 direction
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DNA ligase
seals breaks in the sugar phoshate back bone
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leading strand
The strand of DNA that is synthezied continually
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lagging strand
the other strand which is synthesized in fragments called Okazaki
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Histones
1. DNA condenses in prep of cell division
DNA wrapped around proteins called histones.
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Chromatin
is the genetic term for all of the cells DNA and associated proteins.
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Chromosomes
before cell division each of the individual segments of DNA condenses into these.
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Chromatids
After DNA duplicates there are now two identical segments chomatids "sister".
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centromere
The identical chromatids are attached to each other by a DNA protein complex.
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Prophase(mitosis)
Chromosomes condense
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Miotic Spindle
a specialized cytoskeleton structure that separates the chromatids begins to form(during prohase.
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Mataphase
the membrane around the nucleus breaks down.
the miotic spindle fibers attach to the chromosomes at proteins on the centromeres called kinetochores.
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Anaphase
the miotic spindle pulls apart the chromatids, this breaks the centromeres
the chromatids move to opposite poles of the cell .
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Telophase
The nuclear membrane reforms
the chromosomes decondense
the miotic spindle breaks down
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Mitosis is followed by what?
Cytokinesis the physical separation of the parent cell into two daughter cells This occurs because the cell membrane contracts at the equator until the two new cells are formed.
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