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Gene regulation
- The turning on and off of genes
- Helps organisms respond to environmental changes
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Gene expression
The overall process from genes to proteins or genotype to phenotype
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Promoter
Where RNA polymerase attaches and initiates transcription
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Operator
- DNA control sequence
- Operates as a switch
- Determines whether RNA polymerase can attach to the promoter
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Operon
- Cluster of genes with related functions
- Usually exist only in prokaryotes
- Single on-off switch can control the whole cluster
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Repressor
- Turns off transcription
- Protein that binds to the operator and physically blocks RNA polymerase from attaching
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Regulatory gene
- Outside the operon
- Codes for a repressor protein
- Expressed continually so there's always some repressors
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Activator
- Protein that turns operon on when bound to DNA
- Make it easier for RNA polymerase to attach
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Differentiation
Process cells undergo to become specialized in structure and function
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Histones
Small proteins associated with DNA that deal with DNA packing
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Nucleosome
DNA wound around the protein core of 8 histone molecules
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Linkers
Short pieces of DNA that connect the various nucleosome beads
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Epigenetic inheritance
Inherited traits not directly involving the nucleotide sequence
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X chromosome inactivation
- One of the 2 Xs in females I turned off by compacting one X chromosome
- All daughter cells turn off the same X
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Barr body
Compact object made from an inactivated cell
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Transcription factors
Proteins needed for the RNA polymerase to attach
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Enhancers
- Transcription factors that bind to DNA control sequences
- 1st step to initiate gen transcription
- Cause DNA to bend because Enhancers are far away from genes they regulate
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Silencers
Repressor proteins that bind to DNA sequences to inhibit the start of transcription
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Alternative RNA splicing
More than one polypeptide from each gene due to different combinations of exons- sometimes missing one, sometimes a different, sometimes have all of them
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Micro RNAs
- miRNA
- Small RNA molecules that can bind to complementary mRNA
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RNA interference
- Using miRNA to artificially control gene expression- turn off a certain gene
- RNAi
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Homeotic gene
Master control gene that regulates genes that determine specific anatomy of the body
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DNA microarray
- Glass slide with thousands of different kinds of single strand DNA fragments fixed in tiny wells
- Used to see which genes are active in an organism using fluorescent cDNAs
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Signal transduction pathway
- Converts a signal on a cell's surface to do a specific task inside the cell
- Signal molecule binds to receptor protein in membrane which a activates relay proteins which activates a transcription factor that activates transcription and then translation to produce a specific protein
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Clone
- An organism produced through asexual reproduction that is an exact copy of the parent cell
- Common in plants
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Regeneration
Cells dedifferentiate, divide, the redifferentiate to grow lost limbs
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Oncogene
- Single copies of genes in a cell that can cause cancer
- Greek for tumor
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Proto-oncogene
- Normal, healthy gene with the potential to become an oncogene from:
- Mutation within gene, multiple copies of gene, gene is moved to new DNA locus
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Tumor-suppressor gene
- Prevent in controlled cell division
- Mutation that decreases production can lead to cancer
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Nuclear transplantation
The replacement of a nucleus of an egg cell with the nucleus from an adult somatic cell
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Reproductive cloning
- Implanting the transplanted cell in a surrogate mother
- Birth of new living material
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Embryonic stem cells
- Harvested from a blastocyst
- Give rise to any specialized cell in the body
- Used for therapeutic treatments
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Therapeutic cloning
When the main purpose of embryonic stem cells is for therapeutic treatments
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Adult stem cells
- Unlike embryonic stem cells only give rise to many related cells in the body
- Found in bone marrow, hair, eyes
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Carcinogens
- Cancer-causing agents
- Factors that alter DNA and make cells cancerous
- Promote cell division or genetic mutation
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