Midterm II - Chemical Catalysis

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How many polypeptide chains are present in myoglobin?

1
How many polypeptide chains are present in hemoglobin?

4
How many polypeptide chains are present in IgG?

4
How many polypeptide chains are present in IgM?

21
How many polypeptide chains are present in myosin?

6
How many polypeptide chains are present in G-actin?

1
How many polypeptide chains are present in lysosyme?

1
What is the chemical reaction catalyzed by myosin?

ATP + H20 -> ADP +Pi
What chemical reaction is catalyzed by lysosyme?

(NAM-NAG)n-NAM-NAG-(NAM-NAG)m + H20 -> (NAM-NAG)n-NAM + NAG-(NAM-NAG)m
What chemical reaction is catalyzed by trypsin?

~CHR-CO-NH-CHR'~ + H20 -> ~CHR-COO- + NH3+-CHR'

Where R is Lys (K) or Arg (R)
What chemical reaction is catalyzed by chymotrypsin?

~CHR-CO-NH-CHR'~ + H20 -> ~CHR-COO- + NH3+-CHR'

Where R is Phe (F), Trp (W), or Tyr (Y)
What chemical reaction is catalyzed by alcohol dehydrogenase?

CH3CH2OH + NAD+ -> NADH + H+ +CH3CHO
What chemical reactions are catalyzed by alpha-amylase?

Amylose (amylopectin, glycogen) + H20 -> glucose + maltose
What chemical reactions are catalyzed by Ribonuclease A?

RNA + H2O -> NMPs
What is the catalytic triad found in serine proteases?
- Asp-102
- His-57
- Ser-195
What chemical reactions are catalyzed by carbonic anhydrase?

CO2 +H2O -> COOOH- + H+
What are the active residues in lysozyme?

Glu (E) and Asp (D)
How many steps does RNase A's mechanism consist of? What are the functional amino acid residues?
- Two.
- Two histidines (one protonated, one deprotonated)
What are the following relations for competitive inhibition?
Vmax-app
Km-app
Ki
- Vmax-app = Vmax
- Km-app = a(Km)
- Ki=[I]/(1-a)
What are the following relations for uncompetitive inhibition?
Vmax-app
Km-app
Ki
- Vmax-app = Vmax/a'
- Km-app = Km/a'
- Ki = [I]/(1-a')
What are the following relations for noncompetitive inhibition?
Vmax-app
Km-app
Ki
- Vmax-app = Vmax/a'
- Km-app = (a/a')Km
- Ki = [I]/(1-a)
- Ki' = [I]/(1-a')

Author

Yasham

113242

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Midterm II - Chemical Catalysis

Description

Midterm II - Chemical Catalysis

Updated

2011-10-31T22:01:54Z