In the laboratory, DNA molecules can be cut at specific sequences using..?
Restriction Nucleases
On average, which restriction nuclease would ut a random DNA sequence into a greater number of pieces?
A restriction nuclease that recognizes a four-nucleotide pair sequence.
Gel electrophoresis separates DNA fragments by virtue of which feature?
Size
When a mixture of DNA fragments of different sizes is loaded onto an agarose gel and a voltage is applied, the DNA will migrate in the gel. Which molecules will migrate the fastest?
The shortest.
Nucleic acid hybridization can detect any given DNA or RNA sequence in a misture of nucleic acid fragments. This techniques relies on the fact:
That a single strand of DNA or RNA will form a double helix with another nucleic acid strand of the complementary nucleotide.
What is NOT true about a DNA probe?
(A) it hybridizes only to DNA and not RNA
(B) It contains a nucleotide sequence that is complementary to the nucleic acid sequence of interest
(C) It is typically 10-1000 nucleotides long
(D) It is able to hybridize to a DNA sequence of interest
In Southern Blotting, DNA fragments are first separated from each other by..?
Gel electrophoresis
What does the term DNA cloning refer to?
The act of making many identical copies of a DNA molecule.
Which of the following enzymes allows scientist to join together two DNA fragments?
DNA Ligase
DNA can be introduced into bacteria by a mechanism called...?
Transformation
What does a DNA library consist of?
A collection of cloned DNA fragments in a bacterial culture.
Which of the following DNA libraries lacks introns and regulatory sequences?
(A) A genomic library
(B) A cDNA library
When making a DNA library from a starting material of mRNA, which enzyme is required to copy the mRNA into DNA molecules?
Reverse Transcriptase
Which of the following libraries would be expected to be essentially the same?
(A) Genomic libraries made from mouse liber and kidney cells
(B) cDNA libraries made from mouse liver and kidney cells
(C) Genomic and cDNa libaries made from mouse liver cells
What is the starting material for making a cDNA library?
mRNA
If you want to clone a gene so that you may deduce the amino acid sequence of the protein from the DNA, what type of libary would be most useful to start from?
(A) A genomic library
(B) A cDNA libary
Which of the following is FALSE?
(A) PCR can be used to clone a gene
(B) PCR amplifies a DNA sequence
(C) PCR can be used to sequence a genome
(D) PCR can be used to detect the presence of a virus in a blood sample
In order to design primers for the PCR reaction, which part of the DNA sequence of interest must be known?
The ends of the DNA sequence of interest
In the polymerase chain reation, what is used to separate the two strands of a double-stranded DNA molecules?
high heat
What serves as the original template materal for the PCr reaction?
DNA or RNA
Using the procedure of PCR, after 30 rounds of preplication and beginning with 1 template molecule of dsDNA, how many copies of the target sequence would you expect?
About 1 billion.
What extra sequences are found in expression vectors that are not found in typical vectors?
Promoters
A reporter gene..
Can reveal when a gene is expressed, can reveal where a gene is expressed, and is a gene whose activity can be easily monitored.
Which of the following is considered a reported protein?
Green fluroescent protein
Which of the following techniques allows a researcher to identify the location of particular RNAs in a cell?
In situ hybridization
Which of the following technologies allows us to study the expression patterns of thousands of genes at the same time?
DNA microassays
Cloned DNA can be altered in vitro to create mutant genes that can then be reinserted into a cell or an organism to study gene function.
