Quiz Two Bioprocess

• Physico-chemical properties
• Molecular Size
• Charge
• Solubility
• Hydrophobic and Hydrophillic nature
• Biological Affinity

A process of separating biological molecules from liquid phase by forming an insoluble aggregates

• Aggregates are formed by addition of reagents
• Salt competes for H2O with protein

• A method to aggregate protien by rendering the protein no net electric charge
• Can be done by addition of weak polar solvents or Nonionic polymer

• Obtaining solid forms from impure solution by cooling and vaporization procedure
• Advantage- Single processing step, low energy required
• Disadvantage- non-uniform crystal size

A method of separation of mixtures that exploits the differences in partition behavior

A column chromatography that uses a charged (protiens, amino acids, peptides) stationary phase

A gel permeation chromatography or gel filtration chromatography and separates based on particle size

Based on selective non-covalent interations between an analyte and specific molecules

Compressed gas (usually CO2) as extracting solvent

• It is a process of drying by freezing
• Used for long term storage

Total destruction of all forms of life

Destroy microorganism on inanimate objects

Destroys microorganisms on living tissue

Reduces the number of microbes to a safe level

Kill all microbes

Autoclaving- 160-170 C for > 2 hours

A sterilization technique by moist heat reaching all the surfaces of the material to be sterilized

Using gases and vapors that possess germicidal properties

Implement the use of electrons from accelerators or radioisotopes

• Advantage- good to sterilize istems that dont withstand other types of sterilization
• Disadvantage- corrosion of metal surfaces
• Toxic residual effect on tissue

A technique used to physically exclude microorganisms from a fluid or air