Exam #1 Review Chapter 3

• 1.) Inoculation
• 2.) Isolation
• 3.) Incubation
• 4.) Inspection
• 5.) Information gathering
• 6.) Identification

producing a culture

separating one species from another

Providing proper growth conditions for the organisms to grow and growing the organisms.

viewing organisms macroscopically and microscopically.

any other additional tests to test the organisms.

finding out what you have

• 1. Physical state
• 2. Chemical Composition
• 3. Functional type

• 1. Liquid
• 2. Semisolid
• 3. Solid (Liquidfiable)
• 4. Solid (Non-liquidfiable)

• 1. Synthetic (chemically defined)
• 2. Nonsynthetic (complex; not chemically defined)

• 1. General purpose
• 2. Enriched medium
• 3. Selective media
• 4. Differential media
• 5. Transport media
• 6. Reducing media

• Agar is a liquefiable solid media that is made from red algae.
• It is solid at room temp.
• Melts at 100 degrees C.
• Resolidifies at 42 degrees C.
• Flexible, moldable
• Good framework for nutrients(can add nutrients)
• Indigestible itself

A small sample of organisms.

• Selective media- inhibits growth of most but encourages growth of others. Some organisms grow really well.
• Differential media- allows growth of most but the appearance differs. Appearance may be different for the different organisms. For an example: the color.

• Synthetic media- is all the ingredients are chemically defined meaning that all the ingredients come off the chemistry shelf (examples: sugars, salts, etc.)
• Non-sythetic- at least one of the ingredients is not chemically defined. (Can not be exactly measured) (examples: proteins, yeast extract, blood, beef, milk, soybean, etc.)

Fastidious organisms reguire specialized conditions for growth because they have more needs.

Simmons Citrate agar.

Total Magnification = Objective Magnification X Ocular Magnification

Resolving power is ability to show detail in the organism.

• Scanning electron microscope- gives external surface of microbe( a 3-D image). Has worse magnification of 100,000x and worse resolution of 10 nm than transmission electron microscope.
• Transmission electron microscope- gives fine detail of thinly sliced sections of microbes. Has better magnification of 1,000,000x magnification, and better resolution of 0.5 nm than an scanning electron microscope.

the ability to show detail.

killed

• You are going to see a dark speciman on a light background.
• General purpose use of microscope.

• You are going to see a light specimen on a dark backgrouns.
• Useful on live specimens.

• Varies in the bending of light.
• You are going to see brighter brights and darker darks.
• Good for internal detail.

• Microscope uses ultraviolet light and fluorescent dyes.
• Used to diagnosis Tuberculosis (TB)

• Microscope uses lasers to "slice" specimen.
• Shows good internal detail/ everything is in focus.

A pure culture is growth of one known species.

• A mixed culture is two or more indentified, easily differentiated species.
• (All medical samples would be mixed)

A contaminated culture was intended to be pure or mixed but unknowns ended up being introduced.