Aseptic Technique: Culture Tube Flaming and Inoculation
The cap is removed, and the mouth of the tube is passed through the flame. If the tube is a broth tube, the loop is inserted into the tube and twisted several times to ensure the organisms on the loop are deliverd to the liquid. If the tube is an agar slant, the surface of the slant is inoculated by drawing the loop up the surface of the slant from the bottom of the slant to the top. For stab cultures, a needle is inserted into the agar medium. After the culture is inoculated the mouth if the tube is reflamed, and the tube is recapped.