-
What are some of the requirements for bioreactors? What would make it ideal?
- In vitro environment that contains the following:
- Biochemistry
- Mechanical stimuli
- Promotes and regulates tissue development, maintenance, responses
mimics in vivo
-
What is the ideal bioreactor?
- An amnionic egg that contains all the requirements for the zygote to develop.
- A variation on the ideal bioreactor would be the placenta but they is more of a paraistic relationship.
-
What are the four functions of bioreactors?
- Spatial uniformity
- Maintains gas and nutrient levels
- Provides efficient mass transfer
- Provides appropriate physical stimuli
-
What are two polycarbonate bioreactor designs?
- Scaffold ring
- Integrated cell culture insert
-
Describe the scaffold ring bioreactor design.
Cells are grown on a scaffold ring and then transferred into the bioreactor
-
Describe the integrated cell insert bioreactor design.
Cells are grown on a scaffold integrated into the bioreactor housing, windows with ports are added later for flow.
-
What are some examples of variables that may change in scaffolds?
- Hollow tubes
- Sheets
- 3-D
- Woven or non-woven
- High porosity
- High internal surface area to volume ration
-
What are some methods for assessment of the scaffold?
- Light microscope
- SEM
- TEM
- Freeze fracture
- Atomic force microscope
-
What are some methods for functional assessment?
- Metabolism - glucose consuption and the resultant lactose production
- Cell damage including radiolabel incorporation
- Mechanical properties
-
What are some biodegradable polymers?
- Poly(L-lactic acid)
- Poly(glycolic acid)
- Poly(lactide-co-glycolide)
- Collagen
- GAGs (HA, KS, CS)
- Chitosan
- Polyhydroxyalanoates (PHA)
- Polycaprolactone (PCL)
-
What are some non-biodegradable polymers?
- Polydimethylsioxane (PDMS) aka silicone
- Polyurethanes
- Polytetrafluorethylene
- Polycarbonate
- Polymethyl methacrylate (PMMA)
- Polystyrene
- Polyvinyl pyrrolidone (PVP)
- Polyvinyl alcohol (PVA)
-
What are some ECM additions and their associated functions? (5)
- Glycosaminoglycans (GAGs) - Forms ECM gel
- Collagen - provides strength and organization
- Elastin - provides resilience or elasticity
- Fibronectin - adhesion for cells
- Laminin - adhesion to basal lamina
-
What is the function of glycosaminoglycans (GAGs) as an ECM addition?
Forms ECM gel
-
What is the function of collagen as an ECM addition?
Provides strength and organization
-
What is the function of elastin as an ECM addition?
Provides resilience or elasticity
-
What is the function of fibronectin as an ECM addition?
Adhesion for cells
-
What is the function of laminin as an ECM addition?
Adhesion to basal lamina
-
What are the three types of cell interactions?
- Cell to ECM
- Cell to Cell
- Cell to "factor" (growth, cytokine, chemokine....)
-
What are some applications of tissue engineering?
- Liver
- Dental
- Plastic/reconstructive surgery
- Diabetes
- Site-specific drug delivery
- Parkinson's
- Cornia
- Kidney
- Skin
- Esophagus
- Intestine
- Ureter
- Trachea
- Bladder
- Bone
- Blood
- Muscle
- Tumor destruction
- Blood vessels
- Parathyroid
- Lymphopoiesis
|
|
