2 sizes of genes
1 size of gene
Polymerase Chain Reaction
Denaturing (separating) of the two DNA strands by heating to 90-95
Annealing- tube is cooled to 55-65
C because primers cannot bind at high temperatures. Primers bind to DNA strand.
Elongation- make a complete copy of template. Temperature is raised because Taq polymerase works best at 72
C. Taq polymerase adds nucleotides to primers and eventually makes a copy.
Anneal to complementary DNA bases and determine the fragment of DNA that will be copied.
DNA piece of interest that is copied
Elongates the DNA strand. Adds nucleotides to primers and eventually makes a copy.
form the primer. Complementary base pairing
PCR used for:
Genetic Disease Diagnosis
white at the bottom of gel
more than 2 bands
Causes of no bands:
not enough target DNA
extraction didnt work
What could be causing Eric's problem?
thyroid gland isnt working
pituitary gland has problems
testes didnt mature
adrenal glands are cancerous
is an important connection between the brain and the endocrine glands
Benign pituitary tumor
puts pressure on the pituitary and disrupted its functions
Irradiation to kill the pituitary and any tumor associated
Cartilage operation on his knee/ metal pins in both hips