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Photoautotrophs
- Use light as an energy source.
- Carbon dioxide as carbon source.
- performs photosynthesis.
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What is Transduction?
Dna is moved cell to cell by viruses.
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What is Transformation?
A living cell acquires fragments of DNA from its environment from dead cells and incoporates them into its own cell.
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What is Conjugation?
A small piece of DNA is transferred from a donor cell to a recipient cell, usually through a hollow, tube like connection called a pilus.
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How can prokaryotes move?
- Spirochetes have a corkscrew like motion.
- Some cyanobacteria moe up and down in water by adjusting amount of gas in gas vesicles.
- Most common type is by the flagella.
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Steps of Gram Staining.
- Fixation.
- Crystal Violet.
- Iodine treatment.
- Decolorization.
- Counter stain (safranin).
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What makes a bacteria gram negative?
They have a thin layer of peptidoglycan.
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What makes a bacteria gram positive?
They have a thick layer of peptidoglycan outside the plasma membrane.
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Where can prokaryotes live?
They can live in every habitat.
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What are filaments?
Chains of cells that stayed together after they split.
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What are biofilms? Why are they important?
- they are a gel-like polysaccharide matrix.
- Cells in a biofilm matrix are difficult to kill.
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What are the common shapes of Bacteria?
- Sphere- Coccus (cocci).
- Rod- bacillus (bacilli).
- Spiral- helical (helices).
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Is it easy to trace the evolutionary relationships of bacteria?
- DNA is a problem because of lateral gene transfers.
- Transfer by plasmids or virus, and uptake of DNA by transformation.
- Solution: Need genes from the "stable core" - Nucleotide sequencing of ribosomal RNA is ery useful.
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Obligate aerobes.
Cannot surie in the absence of oxygen.
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Aerotolerant Anaerobes
Do not use oxygen to burn sugar, but are not damaged by oxygen if it is present.
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Anaerobes
- Do not use oxygen to burn sugar.
- Obligate anaerobes - molecular oxygen will kill them.
- Facultative anaerobes- can shift there metabolism between aerobic and anaerobic modes, such as in fermintation.
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Chemoheterotrophs.
- They obtain both energy and carbon from complex organic compounds.
- Most bacteria and archaea are chemoheterotrophs, as are all animals, all fungi, and many protists.
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Chemolithotrophs
Get energy by oxidizing inorganic compounds, and use the energy to fix CO2.
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Photoheterotrophs
Use light as energy source, but get carbon from compounds made by other organisms.
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Prokaryotes Vs Eukaryotes
Differences?
- 1. Prokaryotes divide by binary fission.
- 2. Prokaryotic DNA is a single circular molecule, not in a nucleus.
- 3. Prokaryotes hae no membrane enclosed organelles.
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What does all life have/do?
- Conduct Glycolosis.
- Replicate DNA.
- Have DNA that encodes proteins.
- Produce proteins using the same genetic code.
- Have plasma membranes and ribosomes.
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Spirochetes
- Gram-negatie.
- Motile
- Chemoheterotrophic
- Unique axial filaments that rotate.
- Many are human parasites, some are pathogens, others are free living.
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Chlamydias
- Extremely small Gram negative coccii.
- Live only as parasites in cells of other organisms.
- Can take up ATP from host cell with translocase.
- Complex life cycle.
- Some are pathogens, causing trachoma, sexually transmitted diseases, and pneumonia.
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High GC gram positive
(Actinobacteria)
- High G-C/A ratio in DNA.
- Form elaborately branching filaments.
- Some reproduce by forming chains of spores at tips of filaments.
- Most antibiotics are from this group.
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Cyanobacteria
- Photoautotrophs; many species fix nitrogen.
- Photosynthetic membranes; plant chloroplasts are derived from endosymbiotic cyanobacteria.
- Flat sheets, filaments, to spherical balls of cells.
- Vegetative cells, spores, and heterocysts.
- Heterocysts are specialized for nitrogen fixation.
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Low GC gram positive
- Some produce endospores.
- Endospores become active and divide when conditions improve.
- Closteridium botulinum, Bacillus anthracis, staphylococcus, S aureus.
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Proteobacteria
- Largest group of bacteria-high diversity of metabolic phenotypes.
- Includes some nitrogen-fixing genera.
- E. coli is a proteobacterium.
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How are archeae different?
- Based on gene sequencing.
- Famous for living in extreme environments: High salinity, high temperatures, high or low pH, and low oxygen.
- The long-chain hydrocarbons in archaea are unbranched.
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How do prokaryotes affect their environment?
- Cheese making, sewage treatment, and production of antibiotics, vitamins, and chemicals.
- They metabolize organic compounds in dead organisms and other organic materials.
- Key steps in the cycling of elements.
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Why you should thank a cyanobacteria?
- Cyanobacteria had a large impact on life when they started generative 02 as a by-product of photosynthesis.
- Bacteria in humans produces B12 and K.
- Biofilm that lines human intestines facilitates uptake of nutrients.
- Bacteria are critical in maintaining skin health.
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What is Koch's postulate?
- The microorganism is always found in persons with the disease.
- It can be taken from the host and grown in pure culture.
- A sample of the culture causes the diseases in a new host.
- The new host also yields a pure culture.
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What are two types of bacterial toxins?
- Corynebacterium diptheriae (diptheria) has low invasiveness, but the toxins it produces affect the entire body.
- Bacillus anthracis (anthrax) has alow toxigenicity, but very high invasiveness- it colonizes the entire bloodstream.
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How are viruses put together?
Consist of a nucleic acid core (viral genome) surrounded by a protein coat (capsid).
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