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microscope.txt
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connects base to head-
arm
supports the microscope-
base
used for initial focusing; moves stage up & down-
coarse adjustment knob
takes light coming from substage light & concentrates light on specimen-
condenser
used for precise focusing & focusing at higher magnification; moves stageup & down slightly-
fine adjustment knob
supports ocular & objective lenses-
head
regulates light coming through the condenser-
iris diaphragm
regulates how bright substage light is-
light control or rheostat
holds slide in place; allows for precise movement of specimen-
mechanical stage
how to move mechanical stage-
mechanical stage control
magnify the specimen; each lens has a different magnification-
objective lense
look through to make observation; has magnification of 10x-
ocular lens
carries objective lense; rotates to change objective lense-
rotating nosepiece
supports the slide-
stage
provides light source-
substage light
any speciment being viewed is equal to the power of the ocular lense x the power of the objective-
total magnification
the slide should be in focus at the higher magnifications once you have properly focused in lower magnification-
parfocal
the ability to discriminate 2 close objects as seperate-
resolution
layer that focuses first-
bottom or inferior layer
layer that focuses last-
top or superior layer
formula for calculation FOV-
FOV (low) x total mag (low) = FOV (high) x total mag (high)
ex
: 2mm x 100x = Y x 400x
formula for size of objects-
FOV / # of objects
procedure for preparation of a wet mount-
a)object is placed in a drop of water (or saline) on a clean slide
b)coverslip is held at 45 degree angle with forceps
c)lowered over water & the object
Author
flossie1228
ID
103098
Card Set
microscope.txt
Description
microscope
Updated
2011-09-20T20:26:53Z
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